Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit features:

1. Specific detection of Mycoplasma capricolum and Mycoplasma leachiihas no cross-reactivity with other genomes.

2. High sensitivity, the detection limit is equivalent to 100fg/reaction.

3. The DNA polymerase adopts hot-start mode, which can inhibit non-specific amplification and reduce background fluorescence.

4. This kit comes with a positive control sample, which can be used to test the effectiveness of the kit.

5. This kit comes with ROX reference dye to help correct sampling errors and differences between tubes.

6, with UDG enzyme and dUTP, which can reduce the contamination of residual DNA.

 

Mycoplasma mycoides is a group of infectious mycoplasmas, including six members: Mmc, MmmLC, MmmSC, Mycoplasma leachii, Mcc and Mccp . These members cause a variety of diseases in ruminants ranging from mild to severe and fatal.

Mycoplasma capricolum parasitizes goats , sometimes found in sheep and cattle. Mcc can cause contagious agalactia, polyarthritis, and lung disease in lambs, and Mccp can cause contagious pleuropneumonia in goats. Genetic analysis shows that Mcc and Mccp are very closely related, and they are closely related to Mycoplasma leachii; these three are relatively distantly related to other members of the Mycoplasma mycoides family and can be divided into two groups. groups.

When detecting members of the Mycoplasma mycoides family, cross-reactivity often occurs with serological methods, and The sensitivity is low, and in vitro culture methods are time-consuming. PCR is a highly sensitive and specific detection method that only takes a few hours to obtain results, which can effectively make up for the shortcomings of the above two methods. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

This kit compares the genomes of several members of the Mycoplasma mycoides family. , a common region was selected as a target to specifically identify the Mycoplasma capricolum group, including: Mcc, Mccp, and the closely related Mycoplasma leachii. It was verified by BLAST that there was no cross-reaction with the genomes of other organisms. This kit detects a total of 68 strains of six members of the Mycoplasma mycoides family, Mycoplasma capricolum 24 strains and 7Mycoplasma leachii strains produced specific signals, while the remaining members, as well as 7 other mycoplasma species and 21 bacterial species with similar distribution ranges, did not produce specific signals.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测山羊支原体（包括山羊亚种和山羊肺炎亚种）和Mycoplasma leachii，与其他生物基因组无交叉反应。

2， 灵敏度高，检测极限相当于100fg/反应。

3， DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 本试剂盒带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 本试剂盒带有ROX参比染料，帮助校正加样误差和管间差异。

6， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

 

丝状支原体族（Mycoplasma Mycoides cluster）是一组具有传染性的支原体，包括六个成员： Mmc（Mycoplasma mycoides subsp. Capri.）、MmmLC (Mycoplasma mycoides subsp. mycoides Large Colony，现已被重新归类至Mmc)、MmmSC (Mycoplasma mycoides subsp. mycoides Small Colony) 、Mycoplasma leachii (Mycoplasma sp. bovine group 7, Mbg7)、Mcc (Mycoplasma capricolum subsp. capricolum)和Mccp（Mycoplasma capricolum subsp. capripneumoniae)。这些成员在反刍动物中引起从较温和到严重致死的多种疾病。

山羊支原体（Mycoplasma capricolum）寄生于山羊，有时在绵羊和牛当中也可发现。Mcc可引起传染性无乳症、多发性关节炎和羔羊的肺病， Mccp则引起山羊的传染性胸膜肺炎。遗传分析表明，Mcc和Mccp亲缘关系非常近，二者与Mycoplasma leachii的亲缘关系较近；这三者与丝状支原体族的其他成员亲缘相对较远，可以分为两个群组。

检测丝状支原体族成员时血清学方法常出现交叉反应，且灵敏度较低，而体外培养法又较为耗时。PCR是一种高灵敏度和高特异性的检测方法，仅需几个小时即可获得结果，可以有效弥补上述两种方法的不足。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒通过将几种丝状支原体族成员的基因组进行比较，选取一段共同区域作为靶点特异性识别山羊支原体群组，包括：Mcc、Mccp,以及亲缘很近的Mycoplasma leachii，经BLAST验证，与其他生物基因组无交叉反应。本试剂盒检测了丝状支原体族六个成员共68个菌株，山羊支原体（Mcc、Mccp）24个菌株以及7个Mycoplasma leachii菌株产生特异性信号，其余成员，以及分布范围相近的7种其他支原体和21种细菌，均未产生特异性信号。

 

 

 

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