Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.
 

Mycoplasma conjunctiva is considered to be the main pathogen of infectious keratoconjunctivitis in ruminants such as sheep, goats, and antelopes. IKC is a common infectious eye disease known as pinkeye of small ruminants that can be transmitted between domestic and wild animals and is characterized by inflammation of the conjunctiva and cornea. In advanced stages, the cornea becomes opaque or even perforated, blinding wild animals that may fall off cliffs or die of starvation. One study showed that the positivity rate for Mycoplasma conjunctiva antibodies in adult sheep was 53%. Mycoplasmal IKC can be transmitted between different species, possibly through physical contact or vector insects such as flies. The prevalence of M. conjunctivae is currently unknown in most countries, which may be due to a lack of technology to detect M. conjunctivae.
 

The classic diagnostic method for conjunctival Mycoplasma infection is in vitro culture and subsequent analysis of the mycoplasma colonies Immunological identification.
However, this method is very cumbersome, takes a long time, and requires specialized technology. The use of PCR detection has the characteristics of high sensitivity and high specificity, and the detection time is short, and the results can be obtained in just a few hours.
 

The Mycoplasma conjunctiva PCR detection kit selects a specific sequence of the lipoprotein adhesin gene for PCR identification. The primers have been verified by BLAST to specifically target Mycoplasma conjunctiva and have no cross-reaction with the genomes of other organisms. This kit was used to detect 18 Mycoplasma conjunctiva strains or isolates, as well as 11 other mycoplasmas with close distribution range or close genetic relationship. Only Mycoplasma conjunctiva produced specific amplification bands, and other Mycoplasma species did not have such specific bands. It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma conjunctivalis.


This kit is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

结膜支原体（Mycoplasma Conjunctivae）被认为是绵羊、山羊、羚羊等反刍动物的传染性角膜结膜炎（IKC）的主要病原体。IKC是一种常见的传染性眼病，称为小反刍动物的红眼病，可在家养和野生动物之间传播，其特点是结膜和角膜的炎症。在晚期阶段，角膜变得不透明甚至穿孔，致盲的野生动物可能从悬崖坠落或死于饥饿。一项研究表明，成年绵羊中结膜支原体抗体的阳性率为53％。支原体性IKC可以在不同物种之间传播，传播途径可能是物理接触或苍蝇等媒介昆虫。目前在大多数国家尚不清楚结膜支原体的流行程度，这可能是由于缺乏检测结膜支原体的技术。

结膜支原体感染的经典诊断方法是体外培养和后续对支原体菌落的免疫学鉴定。但是这个方法非常繁琐，需要较长的时间，且需要专业化的技术。而使用PCR法检测则具有高灵敏度和高特异性的特点，且检测时间短，仅需几个小时即可获得结果。

结膜支原体PCR检测试剂盒选取了特异性的脂蛋白粘附素基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向结膜支原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了18个结膜支原体菌株或分离株，以及11种分布范围或亲缘关系较近的其他支原体，仅有结膜支原体产生特异性扩增条带，其他支原体无此特异性条带。可见本试剂盒具有物种特异性，可用于结膜支原体的鉴定和检测。

 本试剂盒仅供科学研究使用。

 

 

 

 

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