Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma Putrefaciens was first discovered in 1954 and confirmed as a new species in 1974. It is an infectious disease recognized by the World Organization for Animal Health. One of the causative agents of breast syndrome. The syndrome is a global disease affecting the mammary glands, joints and eyes, and occasionally causes respiratory disease in sheep and goats. Mastitis can be induced by intramammary inoculation of less than 50 Mycoplasma putrefactive cells into lactating goats. Mycoplasma putrefactiveis is closely related to the Mycoplasma mycoides family. Its unique feature is that it emits a rancid smell during the culture process. The reason may be that its cells contain a pyridoxal phosphate-dependent enzyme. The reaction product of this enzyme is methylmercaptan. , has a unique smell. Mycoplasma putrefae has a genome size of about 832kb, a G+C content of 26.9%, contains 725 genes, and cannot hydrolyze arginine or liquefy coagulated serum.
 

In vitro culture methods, serological methods and PCR methods can all be used to detect putrefactive Mycoplasma . In vitro culture method is a classic detection method, but it is time-consuming and serological method has low sensitivity. The PCR method has the advantages of high sensitivity and strong specificity, and the detection time is short, and the results can be obtained in just a few hours.
 

The Mycoplasma putrefactive PCR detection kit selects the ornithine formyltransferase gene A sequence was identified by PCR, and the primers were verified to be specific targets by BLAST.Mycoplasma putrefaciens has no cross-reactivity with the genomes of other organisms. This kit was used to detect 12 Mycoplasma putrefactive strains and 10 other mycoplasma species that are closely related or distributed . Only each strain of Mycoplasma putrefans produced specific bands. It can be seen that this kit is species-specific and can be used for the identification and detection of putrefactive Mycoplasma.
 

This kit is for scientific research only.

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

腐败支原体（Mycoplasma Putrefaciens）首次发现于1954年，于1974年被确认为新物种，是世界动物卫生组织认可的传染性无乳综合症的病原体之一。这种综合症是一种全球性疾病，影响乳腺、关节和眼睛，偶尔会引起绵羊和山羊的呼吸系统疾病。将少于50个腐败支原体细胞通过乳房内接种到泌乳山羊即可诱导产生乳房炎。腐败支原体与丝状支原体族亲缘关系较近，其独特之处是在培养过程中会发出腐臭气味，原因可能是其细胞内含有磷酸吡哆醛依赖性酶，该酶的反应产物是甲硫醇，具有独特的气味。腐败支原体的基因组大小约832kb，G + C含量为26.9％，含有725个基因，不能水解精氨酸，也不能液化凝结的血清。

体外培养法、血清学方法和PCR法均可用于检测腐败支原体。体外培养法是经典的检测方法，但耗时较长，而血清学方法灵敏度较低。PCR法则有灵敏度高和特异性强的优点，且检测时间短，仅需几个小时即可获得结果。

腐败支原体PCR检测试剂盒选取了鸟氨酸甲酰基转移酶基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向腐败支原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了12个腐败支原体菌株，以及其他10种亲缘关系或分布范围较近的支原体（共92个菌株），仅有腐败支原体各菌株产生特异性条带。可见本试剂盒具有物种特异性，可用于腐败支原体的鉴定和检测。

 本试剂盒仅供科学研究使用。

 

 

 

 

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