Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma floccosum without cross-reaction with other biological genomes.

2. High sensitivity, the lowest detection limit is 80 copies in the reaction solution.

3. The DNA polymerase used has the characteristics of strong anti-inhibition ability and good thermal stability; using hot start method, it can Inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.

Mycoplasma floccosum is a commensal bacterium in the porcine respiratory mucosa. It can be found in the lungs and nasal cavity. According to antigen composition and genome analysis, it is related to Mycoplasma hyopneumoniae . The relationship is very close and often interferes with its detection. Mycoplasma hyopneumoniae is the pathogen of endemic pneumonia in pigs, while Mycoplasma hyopneumoniae is generally non-pathogenic and may adhere to ciliated epithelial cells but does not destroy the cells. It sometimes causes opportunistic infections when Mycoplasma hyopneumoniae invades the body. These two mycoplasmas have characteristics that differ from most mycoplasma species in that they are difficult to culture and do not have the typical "fried egg" appearance of colonies on solid media. The size and shape of Mycoplasma floccosum are not fixed, especially in the later stages of growth. In the stable and declining stages of growth, filamentous cells can be found, a cell shape that is not present in M. hyopneumoniae.

Because Mycoplasma floccosum does not cause disease, although it is widely distributed in pigs, there are few detection methods. The main method is in vitro culture, which is time-consuming. The PCR method can be used to detect Mycoplasma floccosum quickly. , has the advantages of strong specificity and high sensitivity, and the results can be obtained in just a few hours. Ordinary PCR experimental operations are more cumbersome and can only conduct qualitative analysis of target DNA. In contrast, real-time quantitative PCR can not only conduct qualitative analysis of target DNA For quantitative analysis, the experimental steps are also relatively simple, with higher sensitivity and less affected by environmental pollution.

Probe method Mycoplasma floccosum quantitative PCR kit selects a membrane transporter gene as the target. It has been verified by BLAST that it specifically targets Mycoplasma floccosum and has no cross-reaction with other biological genomes. Use this kit 24 species of mycoplasma and 10 species of bacteria that were closely related or distributed were detected. Only Mycoplasma floccosum could produce a specific signal, and no cross-reaction occurred in the rest. 141 samples were tested and 25 positive, which is completely consistent with the ordinary PCR test results.

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测絮状支原体，与其他生物基因组无交叉反应。

2， 灵敏度高，最低检测极限为反应液中80个拷贝。

3， 使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

絮状支原体（Mycoplasma Flocculare）是猪呼吸道粘膜的共生菌，在肺和鼻腔均可发现，根据抗原组合物和基因组分析判断，和猪肺炎支原体（Mycoplasma hyopneumoniae）亲缘关系非常近，常对其检测造成干扰。猪肺炎支原体是猪的地方性肺炎的病原体，而絮状支原体一般是非致病性的，可能是粘附在纤毛上皮细胞上，但不破坏细胞，在猪肺炎支原体侵袭机体时有时造成机会感染。这两种支原体有着与大多数支原体不同的特征，即很难培养和在固体培养基上的菌落外观不是典型的“煎蛋”样子。絮状支原体大小和形状不是固定不变的，特别是在生长后期。在生长稳定和趋向下降阶段，可发现细丝状细胞，这种细胞形状在猪肺炎支原体中不存在。

由于絮状支原体不引起疾病，虽然在猪群中分布广泛，但检测方法很少，主要还是体外培养法，耗时较长。使用PCR法可以对絮状支原体进行检测，有速度快、特异性强、灵敏度高的优势，仅需几个小时即可获得结果。普通PCR实验操作较为繁琐，只能对目标DNA进行定性分析，相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高，更少受环境污染的影响。

探针法絮状支原体定量PCR试剂盒选取一个膜转运蛋白基因作为靶点，经BLAST验证，特异性靶向絮状支原体，与其他生物基因组无交叉反应。使用本试剂盒检测了亲缘关系或分布范围较近的24种支原体（包括猪肺炎支原体）和10种细菌，只有絮状支原体可以产生特异性信号，其余均无交叉反应发生。对141个样品进行检测，得到25个阳性，与普通PCR法检测结果完全一致。

 

 

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