Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma hyopneumoniae, no cross-reaction with other biological genomes.

2. High sensitivity, the lowest detection limit is 5 genomes in the reaction solution.

3. Use hot-start Taq enzyme to inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5. With dUTP and UDG enzymes, it can reduce the contamination of residual DNA.

Mycoplasma hyopneumoniae is the main pathogen of endemic swine pneumonia . Swine pneumonia is a chronic respiratory disease characterized by high morbidity and low mortality that occurs worldwide. Mycoplasma hyopneumoniae causes stunted growth, poor food conversion, dry cough, and increased susceptibility to other biological infections in pigs. Mycoplasma hyopneumoniae attaches to the cilia of pig lung epithelial cells, causing cilia to stagnate, agglomerate and lose, and ultimately lead to epithelial cell death, which is the cause of lung damage and lesions in pigs. This damage prevents normal ciliary clearance and often triggers secondary infection. The immune response of pigs to Mycoplasma hyopneumoniae is slow and ineffective. This mycoplasma does not produce any particularly harmful toxins, but some metabolic by-products of minimal toxicity are observed.

For the detection of Mycoplasma hyopneumoniae, in vitro culture and immunofluorescence methods are often used . due to swine pneumoniaMycoplasma growth conditions are very finicky, and both detection methods sometimes take up to a month to complete. Due to its slow growth, other mycoplasmas, especially Mycoplasma hyorhinis, often contaminate the culture of Mycoplasma hyopneumoniae, so the culture method is rarely used. Due to the slow response of the pig immune system, serological detection methods are often ineffective and may cross-react with Mycoplasma hyorhinis and Mycoplasma flocculare . The PCR method is used for detection, which has higher sensitivity and specificity, and the detection time is short, and the results can be obtained in just a few hours. Ordinary PCR experiments are more cumbersome and can only conduct qualitative analysis of target DNA. In contrast, real-time quantitative PCR can not only conduct quantitative analysis of target DNA, but the experimental steps are also simpler, more sensitive, and less susceptible to environmental contamination. Influence.

Probe method Mycoplasma hyopneumoniae quantitative PCR kit selects an adhesion protein gene As a target, it has been verified by BLAST that it specifically targets Mycoplasma hyopneumoniae and has no cross-reactivity with other biological genomes. This kit was used to detect 11 species of mycoplasma and 10 species of bacteria that are closely related or distributed. Only Mycoplasma hyopneumoniae can produce specific signals, and no cross-reaction occurs in the rest. Fifty-two respiratory swabs and lavage fluid samples taken 28 days after M. hyopneumoniae inoculation were tested and 51 were positive.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1，  特异性检测猪肺炎支原体，与其他生物基因组无交叉反应。

2，  灵敏度高，最低检测极限为反应液中5个基因组。

3，  使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。

4，  带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5，  带有dUTP和UDG酶，可降低残留DNA的污染。

 

猪肺炎支原体（Mycoplasma Hyopneumoniae）是地方性猪肺炎（又称猪支原体性肺炎、猪气喘病）的主要病原体。猪肺炎是一种慢性呼吸系统疾病，以高发病率和低死亡率为特征，在世界各地均可发生。猪肺炎支原体会造成猪发育迟缓，食物转化不良，干咳，对其他生物感染的易感性增加。猪肺炎支原体附着于猪肺上皮细胞的纤毛上，导致纤毛停滞、结块和丢失，最终导致上皮细胞死亡，这是猪肺部损伤病变的原因。这种损害妨碍正常的纤毛清除，并且经常引发继发性感染。猪对猪肺炎支原体的免疫应答缓慢且无效。这种支原体不产生任何特别有害的毒素，但是可观察到一些毒性很小的代谢副产品。

对猪肺炎支原体的检测，常采用体外培养法和免疫荧光法。由于猪肺炎支原体生长条件非常挑剔，这两种检测方法有时需要一个月才能完成。由于生长很慢，其他支原体，尤其是猪鼻支原体（Mycoplasma Hyorhinis），常对猪肺炎支原体的培养造成污染，因此培养法很少使用。由于猪免疫系统应答缓慢，血清学检测方法往往无效，而且会与猪鼻支原体和絮状支原体(Mycoplasma flocculare)产生交叉反应。使用PCR法检测，具有更高的灵敏度和特异性，且检测时间短，仅需几个小时即可获得结果。普通PCR实验操作较为繁琐，只能对目标DNA进行定性分析，相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高，更少受环境污染的影响。

探针法猪肺炎支原体定量PCR试剂盒选取一个粘附蛋白基因作为靶点，经BLAST验证，特异性靶向猪肺炎支原体，与其他生物基因组无交叉反应。使用本试剂盒检测了亲缘关系或分布范围较近的11种支原体和10种细菌，只有猪肺炎支原体可以产生特异性信号，其余均无交叉反应发生。对猪肺炎支原体接种28天后的52个呼吸道拭子和灌洗液样品进行检测，得到51个阳性。

 

 

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