Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma suis. When preparing samples, care must be taken to avoid mixing with bovine blood products.

2. Use hot-start Taq enzyme to inhibit non-specific amplification and reduce background fluorescence.

3, with positive control sample, which can be used to test the effectiveness of the kit and verify the sample to be tested Does it contain PCR inhibitors?

4, with ROX reference dye to help correct sampling errors and differences between tubes.

5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.

Common parasitism in pigs Mycoplasma species include the following: Mycoplasma suis, Mycoplasma hyopneumoniae, Mycoplasma floccosum, Mycoplasma hyorhinis and Mycoplasma hyorhinis . Mycoplasma suis, also known as Eperythrozoon Suis, lives in the blood and is classified as a hemophilic mycoplasma.

Haemotropic Mycoplasma is a type of bacteria that lives on the surface of red blood cells. It has no cell wall and has Polymorphism, which can be transmitted through blood, was once named Hemobartonella and Eperythrozoon . According to 16S rRNA gene sequence analysis, Reclassified as Mycoplasma. There are three types of haemophilic mycoplasmas that have been found in pigs: Mycoplasma suis, Mycoplasma parvum and Candidatus Mycoplasma haemosuis .

 

Mycoplasma suis was formerly known as Eperythrozoon suis), first reported in 1932, is rod-shaped, pleomorphic, and is the causative agent of porcine eperythrozoidosis. Symptoms after pig infection are diverse. During an acute attack, sows will show symptoms such as anemia, jaundice, and anorexia for 1-3 days, which may be accompanied by fever, decreased milk production, and abnormal maternal behavior; piglets will show severe anemia and fever. . After the acute attack, it enters the chronic infection stage, which is characterized by slow growth, susceptibility to disease, and decreased reproductive capacity. Experiments have shown that Mycoplasma suis is directly related to reproductive problems in sows.

There is no suitable in vitro culture method for Mycoplasma suis, and the detection method is generally Giemsa. Stained blood smear method. The detection sensitivity and specificity of the blood smear method are relatively poor, and it is easily interfered by the preparation method and other components in the blood. In the acute attack stage, the bacteria are not yet obvious and cannot be detected until the disease progresses further. Serological methods are rarely used due to difficulty in obtaining sufficient antigens. The PCR method is more convenient and more sensitive, and the detection time is short, and the results can be obtained in just a few hours.

This kit uses dye method real-time quantitative PCR and is designed specifically for the 16S rRNA gene The specific primer can accurately identify Mycoplasma suis and has only weak cross-reactivity with the Mycoplasma villonigensis genome. Mycoplasma villonii lives in bovine blood, so please note when preparing samplesBe careful to avoid mixing with bovine blood products. Blood samples from 120 pigs were tested and the positive rate for Mycoplasma suis was 7.5%. It can be seen that this kit can be used for the detection and identification of Mycoplasma suis.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测猪支原体，准备样品时需注意避免混入牛血制品。

2， 使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。

3， 带有阳性对照样品（组分C），可用于检验试剂盒有效性，以及验证待测样品中是否含有PCR抑制剂。

4， 带有ROX参比染料，帮助校正加样误差和管间差异。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

常见的寄生于猪的支原体有如下几种：猪支原体（Mycoplasma suis），猪肺炎支原体（Mycoplasma hyopneumoniae），絮状支原体（Mycoplasma flocculare），猪鼻支原体（Mycoplasma hyorhinis）和猪滑液支原体（Mycoplasma Hyosynoviae）。猪支原体也称为猪附红细胞体（Eperythrozoon Suis），寄生于血液，在分类上属于嗜血支原体。

嗜血支原体（Hemotropic Mycoplasma，或hemoplasma)是一类寄生于红细胞表面的细菌，无细胞壁，具多形性，可通过血液传播，曾被命名为血巴通氏体（Hemobartonella）和附红细胞体（Eperythrozoon），根据16S rRNA基因序列分析，被重新归类为支原体。在猪体内已发现的嗜血支原体有三种：猪支原体、小支原体（Mycoplasma parvum）和Candidatus Mycoplasma haemosuis（存在与否尚存争议）。

猪支原体旧称猪附红细胞体（Eperythrozoon suis），最早报道于1932年，呈杆状，具多形性，是猪附红细胞体病的病原体。猪感染后症状表现多样，急性发作时，母猪表现出贫血、黄疸和厌食等症状1-3天，同时可能伴随发烧和产奶量下降，以及母性行为异常；仔猪则表现为严重贫血和发烧。急性发作后进入慢性感染阶段，此时表现为生长缓慢、易患病、繁殖能力下降。有实验表明，猪支原体和母猪的生殖问题直接相关。

猪支原体尚无合适的体外培养方法，检测方法一般采用吉姆萨染色的血涂片法。血涂片法的检测灵敏度和特异性均比较差，容易受到制片方法和血液里其他成分的干扰。在急性发作期，此时菌体尚不明显，需病程进一步发展才能检测。血清学方法由于难以获得足够的抗原，所以很少得到应用。而使用PCR法检测则更为方便，且灵敏度更高，检测时间短，仅需几个小时即可获得结果。

本试剂盒采用染料法实时定量PCR，针对16S rRNA基因设计特异性引物，可准确识别猪支原体，仅与维容氏支原体基因组有弱交叉反应。维容氏支原体寄生于牛血中，在准备样品时需注意避免混入牛血制品。用120只猪的血样进行测试，猪支原体的阳性率为7.5%。可见本试剂盒可用于猪支原体的检测和鉴定。

 

 

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