Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma in porcine synovial fluid without cross-reaction with other biological genomes.

2. High sensitivity, the detection limit is equivalent to 10 copies/reaction.

3. Use hot-start DNA polymerase to inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5, with ROX reference dye to help correct sampling errors and differences between tubes.

6, with UDG enzyme and dUTP, which can reduce the contamination of residual DNA.

Mycoplasma porcine synovial fluid is widely distributed in pigs around the world and is easily transmitted in infected pigs. It is excreted through respiration, saliva and feces and spreads rapidly in the form of droplets, sometimes without causing clinical symptoms. Sometimes serious illness can result. Mycoplasma suis synoviae is a commensal of the tonsils and upper respiratory tract of many pigs, and these pigs may become lifelong carriers. Mycoplasma suis synovialis can circulate in the blood and has an affinity for joint tissue, infecting joints and tendon sheaths, causing non-suppurative arthritis, resulting in obvious lameness and pain. It is easy to cause disease in growing pigs aged 8-30 weeks, but it is very rare in older sows because the immune resistance produced by the pig body has already formed. This immune resistance can be passed on to offspring through colostrum and is passed on in older sows. Gradually disappears within weeks.

 

Both in vitro culture methods and PCR methods can be used to detect Mycoplasma in porcine synovial fluid. Compared with culture methods and serological detection methods, the PCR method has the advantages of fast speed, strong specificity, and high sensitivity. Ordinary PCR experiments The operation is more cumbersome and can only perform qualitative analysis of target DNA. In contrast, real-time quantitative PCR can not only perform quantitative analysis of target DNA, but also has simpler experimental steps, higher sensitivity, and is less affected by environmental pollution.

 

This kit selects the 16S rRNA gene as the target and specifically identifies porcine synovial fluid Mycoplasma, verified by BLAST, has no cross-reactivity with other biological genomes. This kit detects a total of 70 strains of four common porcine parasitic mycoplasmas, as well as other 7 mycoplasma and 11 bacterial species with similar distribution ranges. Only porcine synovial fluid All 12 strains of Mycoplasma produced specific signals, and the rest did not produce specific signals.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测猪滑液支原体，与其他生物基因组没有交叉反应。

2， 灵敏度高，检测极限相当于10个拷贝/反应。

3， 采用热启动DNA聚合酶，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有ROX参比染料，帮助校正加样误差和管间差异。

6， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

猪滑液支原体（Mycoplasma Hyosynoviae）在全世界的猪群中广泛分布，易于在感染的猪体内传播，通过呼吸、唾液和粪便排出体外，以液滴形式迅速传播，有时不引起临床症状，有时会导致严重的疾病。猪滑液支原体是许多猪的扁桃体和上呼吸道共生菌，这些猪可能成为其终生携带者。猪滑液支原体可以在血液中循环，对关节组织有亲和力，感染关节和肌腱鞘，引起非化脓性关节炎（猪滑液支原体关节炎），导致明显的跛行和疼痛。在8-30周龄的生长猪身上易引起疾病，在较老的母猪中非常罕见，因为猪体对其产生的免疫抵抗已经形成，这种免疫抵抗可通过初乳传给后代，并在数周内逐渐消失。

体外培养法和PCR法均可用于检测猪滑液支原体。与培养法和血清学检测方法相比，PCR法具有速度快、特异性强、灵敏度高的优势。普通PCR实验操作较为繁琐，只能对目标DNA进行定性分析，相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高，更少受环境污染的影响。

本试剂盒选取16S rRNA基因作为靶点，特异性识别猪滑液支原体，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了四种常见的猪寄生性支原体共70个菌株，以及分布范围相似的其他7种支原体和11种细菌，只有猪滑液支原体的全部12个菌株产生特异性信号，其余均未产生特异性信号。

 

 

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