Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma hyopneumoniae is the main pathogen of endemic swine pneumonia . Swine pneumonia is a chronic respiratory disease characterized by high morbidity and low mortality that occurs worldwide. Mycoplasma hyopneumoniae can cause growth retardation, poor food conversion, and increased susceptibility to other biological infections in pigs. Mycoplasma hyopneumoniae attaches to the cilia of pig lung epithelial cells, causing cilia to stagnate, agglomerate and lose, and ultimately lead to epithelial cell death, which is the cause of lung damage and lesions in pigs. This damage prevents normal ciliary clearance and often triggers secondary infection. The immune response of pigs to Mycoplasma hyopneumoniae is slow and ineffective. This mycoplasma does not produce any particularly harmful toxins, but some metabolic by-products of minimal toxicity are observed.

For the detection of Mycoplasma hyopneumoniae, in vitro culture methods and immunofluorescence methods are often used. Since the growth conditions of Mycoplasma hyopneumoniae are very picky, these two detection methods sometimes take a month to complete. Due to the slow growth , other mycoplasmas, especially Mycoplasma hyorhinis, often cause contamination in the culture of Mycoplasma hyopneumoniae. Serological detection methods are also related to Mycoplasma hyorhinis and Mycoplasma flocculare produces cross-reaction. The use of PCR detection has higher sensitivity and specificity, and the detection time is short, and the results can be obtained in just a few hours.

The Mycoplasma hyopneumoniae PCR detection kit selects a cytoplasmic protein gene sequence with lactate dehydrogenase activity for PCR identification. This gene Highly conserved and species-specific. The primers were verified by BLAST to specifically target Mycoplasma hyopneumoniae and have no cross-reactivity with the genomes of other organisms. This kit was used to detect 16 common mycoplasmas and Gram-negative and positive species in the porcine respiratory tract. Bacteria, only Mycoplasma hyopneumoniae produces specific amplification bands, and the others have no cross-reactivity. It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma hyopneumoniae.

This kit is for scientific research only.

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

猪肺炎支原体（Mycoplasma Hyopneumoniae）是地方性猪肺炎（又称猪支原体性肺炎、猪气喘病）的主要病原体。猪肺炎是一种慢性呼吸系统疾病，以高发病率和低死亡率为特征，在世界各地均可发生。猪肺炎支原体会造成猪发育迟缓，食物转化不良，对其他生物感染的易感性增加。猪肺炎支原体附着于猪肺上皮细胞的纤毛上，导致纤毛停滞、结块和丢失，最终导致上皮细胞死亡，这是猪肺部损伤病变的原因。这种损害妨碍正常的纤毛清除，并且经常引发继发性感染。猪对猪肺炎支原体的免疫应答缓慢且无效。这种支原体不产生任何特别有害的毒素，但是可观察到一些毒性很小的代谢副产品。

对猪肺炎支原体的检测，常采用体外培养法和免疫荧光法。由于猪肺炎支原体生长条件非常挑剔，这两种检测方法有时需要一个月才能完成。由于生长很慢，其他支原体，尤其是猪鼻支原体（Mycoplasma Hyorhinis），常对猪肺炎支原体的培养造成污染。血清学检测方法也会与猪鼻支原体和絮状支原体(Mycoplasma flocculare)产生交叉反应。而使用PCR法检测，具有更高的灵敏度和特异性，且检测时间短，仅需几个小时即可获得结果。

猪肺炎支原体PCR检测试剂盒选取了一个有乳酸脱氢酶活性的胞质蛋白基因序列进行PCR鉴定，该基因高度保守，且具有物种特异性。引物经BLAST验证为特异性靶向猪肺炎支原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了16种猪呼吸道常见的支原体和革兰氏阴性、阳性细菌，仅有猪肺炎支原体产生特异性扩增条带，其他均无交叉反应。可见本试剂盒具有物种特异性，可用于猪肺炎支原体的鉴定和检测。

本试剂盒仅供科学研究使用。

 

 

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