SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 20μL;  DMEM cell culture supernatant: 100μL
SENSITIVITY	3.03 pg/mL
RANGE	31.25 pg/mL – 2000 pg/mL
ASSAY TIME	1.5 h
RECOVERY	85% – 112%
AVERAGE RECOVERY	0.94
INTRA PRECISION	4.1% – 6.4%
INTER-PRECISION	2.1% – 2.6%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF monoclonal antibody Human VEGF freeze-dried standard VEGF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human VEGF has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for VEGF (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then VEGF present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of VEGF bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader.

 

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