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One Step ELISA Kit For Human Vascular Endothelial Growth Factor A (VEGFA)
One Step ELISA Kit For Human Vascular Endothelial Growth Factor A (VEGFA)
VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
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  • NO.:One-EK002H
    Validity:Two Years
    Assay type:One step sandwich elisa
    Species:Human
    Sensitivity:3.03 pg/mL
    Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
    Assay Time:1.5 hours
    Assay range:31.25pg/mL–2000pg/mL
    NCBI_Gene:7422
    UniProtKB:P15692
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Details

SAMPLE TYPE Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME Serum or plasma: 20μL;  DMEM cell culture supernatant: 100μL
SENSITIVITY 3.03 pg/mL
RANGE 31.25 pg/mL – 2000 pg/mL
ASSAY TIME 1.5 h
RECOVERY 85% – 112%
AVERAGE RECOVERY 0.94
INTRA PRECISION 4.1% – 6.4%
INTER-PRECISION 2.1% – 2.6%
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF monoclonal antibody
Human VEGF freeze-dried standard
VEGF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human VEGF has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for VEGF (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then VEGF present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of VEGF bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader.

 

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