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One Step ELISA Kit For Human Interleukin 10 (IL10)
One Step ELISA Kit For Human Interleukin 10 (IL10)
IL-6,CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
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  • NO.:One-EK004H
    Validity:Two Years
    Assay type:One step sandwich elisa
    Species:Human
    Assay range:15.63 pg/mL – 1000 pg/mL
    Sensitivity:11.71 pg/mL
    Sample Volume:Serum or plasma: 10μL; DMEM cell culture supernatant: 100μL
    Assay Time:1.5 hours
    NCBI_Gene:3586
    UniProtKB: P22301
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Details

SAMPLE TYPE Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME Serum or plasma: 10μL; DMEM cell culture supernatant: 100μL
SENSITIVITY 11.71 pg/mL
RANGE 15.63 pg/mL – 1000 pg/mL
ASSAY TIME 1.5 h
RECOVERY 90% – 107%
AVERAGE RECOVERY 0.91
INTRA PRECISION 3.9% – 5.7%
INTER-PRECISION 4.5% – 7.3%
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL10 monoclonal antibody
Human IL10 freeze-dried standard
IL10 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human IL-10 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-10 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-10 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-10 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader

 

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