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One Step ELISA Kit For Human Interleukin 6 (IL6)
One Step ELISA Kit For Human Interleukin 6 (IL6)
IL-6,MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
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  • NO.:One-EK005H
    Validity:Two Years
    Assay type:One step sandwich elisa
    Species:Human
    Assay range:1.56 pg/mL – 100 pg/mL
    Sensitivity:1.05 pg/mL
    Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
    Assay Time:1.5 hours
    NCBI_Gene:3569
    UniProtKB: P05231
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SAMPLE TYPE Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
SENSITIVITY 1.05 pg/mL
RANGE 1.56 pg/mL – 100 pg/mL
ASSAY TIME 1.5 h
RECOVERY 76% – 125%
AVERAGE RECOVERY 1.00
INTRA PRECISION 7.1%-9.5%
INTER-PRECISION 2.8% – 9.2%
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody Human IL-6 freeze-dried standard IL-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilized
antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

 

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