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One Step ELISA Kit For Mouse Tumor Necrosis Factor Alpha (TNFa)
One Step ELISA Kit For Mouse Tumor Necrosis Factor Alpha (TNFa)
DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
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  • NO.:One-EK006M
    Assay Time:1.5 hours
    Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
    Sensitivity:1.70 pg/mL
    Species:Mouse
    Validity:Two Years
    Assay type:One step sandwich elisa
    Assay range:10.938pg/mL–700pg/mL
    NCBI_Gene:7124
    UniProtKB:P01375
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SAMPLE TYPE Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
SENSITIVITY 1.70 pg/mL
RANGE 10.938 pg/mL – 700 pg/mL
ASSAY TIME 1.5 h
RECOVERY 81% – 112%
AVERAGE RECOVERY 0.95
INTRA PRECISION 3.1% – 3.7%
INTER-PRECISION 2.3% – 3.1%
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody
Mouse TNF-α freeze-dried standard
TNF-α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse TNF-α has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for TNF-α (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then TNF-α present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TNF-α bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

 

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