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One Step ELISA Kit For Mouse Interleukin 18 (IL18)
One Step ELISA Kit For Mouse Interleukin 18 (IL18)
IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma
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  • NO.:One-EK007M
    Assay Time:1.5 hours
    Species:Mouse
    Validity:Two Years
    Assay type:One step sandwich elisa
    Sample Volume: Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
    Sensitivity:0.35 pg/mL
    Assay range:3.125pg/mL–200pg/mL
    NCBI_Gene:3606
    UniProtKB:Q14116
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SAMPLE TYPE Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
SENSITIVITY 0.35 pg/mL
RANGE 3.125 pg/mL – 200 pg/mL
ASSAY TIME 1.5 h
RECOVERY 88% – 114%
AVERAGE RECOVERY 0.99
INTRA PRECISION 3.4% – 4.6%
INTER-PRECISION 3.1% – 5.4%
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL18 monoclonal antibody
Human IL18 freeze-dried standard
IL18 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

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