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One Step ELISA Kit For Human/Mouse/Rat Transforming Growth Factor beta 1 (TGF-β1)
One Step ELISA Kit For Human/Mouse/Rat Transforming Growth Factor beta 1 (TGF-β1)
Tgfb1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP; Transforming growth factor beta-1 ELISA Kit; TGF-beta-1 ELISA Kit
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  • NO.:One-EK008Mt
    Assay Time:1.5 hours
    Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
    Sensitivity:9.20 pg/mL
    Assay range:15.63pg/mL-1000pg/mL
    Validity:Two Years
    Assay type:One step sandwich elisa
    Species:Human/Mouse/Rat
  • Goods click count:438
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SAMPLE TYPE Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
SENSITIVITY 9.20 pg/mL
RANGE 15.63 pg/mL - 1000 pg/mL
ASSAY TIME 1.5 h
RECOVERY 88% – 114%
AVERAGE RECOVERY 0.99
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TGF-β1 monoclonal antibody TGF-β1 freeze-dried standard
TGF-β1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for TGF-β1 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for TGF-β1 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then TGF-β1 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TGF-β1 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

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