Summary:

 

Purpose

 

Our Quick Step Rat myeloperoxidase, MPO ELISA Kit (Enzyme activity) is to assay MPO levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to MPO. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for MPO is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MPO and HRP conjugated MPO antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MPO. You can calculate the concentration of MPO in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0508Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0U/ml	0.044	0.0480
		0.052
S1	60U/ml	1.999	1.9865
		1.974
S2	40U/ml	1.302	1.3115
		1.321
S3	20U/ml	0.687	0.6900
		0.693
S4	10U/ml	0.392	0.3855
		0.379
S5	5U/ml	0.202	0.2030
		0.204

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