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Summary:
Purpose
Our Quick Step Rat Matrix Metalloproteinase 2/Gelatinase A (MMP-2)ELISA Kit is to assay MMP-2 levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to MMP-2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for MMP-2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MMP-2 and HRP conjugated MMP-2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MMP-2. You can calculate the concentration of MMP-2 in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QS0483Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Standard
Concentration
OD Value
Average OD Value
Blank Well
0ng/ml
0.060
0.0560
0.052
S1
7.2ng/ml
1.960
1.9575
1.955
S2
4.8ng/ml
1.292
1.2875
1.283
S3
2.4ng/ml
0.701
0.7045
0.708
S4
1.2ng/ml
0.391
0.3920
0.393
S5
0.6ng/ml
0.212
0.2045
0.197
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