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Summary:
Purpose
Our Quick Step Rat Cytochrome Oxidase 4 (COX4)ELISA Kit is to assay COX4 levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to COX4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for COX4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain COX4 and HRP conjugated COX4 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of COX4. You can calculate the concentration of COX4 in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QSD1885Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Standard
Concentration
OD Value
Average OD Value
Blank Well
0ng/L
0.044
0.0480
0.052
S1
60ng/L
1.999
1.9865
1.974
S2
40ng/L
1.302
1.3115
1.321
S3
20ng/L
0.687
0.6900
0.693
S4
10ng/L
0.392
0.3855
0.379
S5
5ng/L
0.202
0.2030
0.204
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