Summary:

 

Purpose

 

Our Quick Step Rat Complement 3a (C3a)elisa Kit is to assay C3a levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to C3a. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for C3a is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain C3a and HRP conjugated C3a antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of C3a. You can calculate the concentration of C3a in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1176Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.043	0.0475
		0.052
S1	72pg/ml	1.988	1.9805
		1.973
S2	48pg/ml	1.302	1.3095
		1.317
S3	24pg/ml	0.692	0.7035
		0.715
S4	12pg/ml	0.377	0.3855
		0.394
S5	6pg/ml	0.202	0.1980
		0.194

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