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Drug Names
Generic Name: Human RHO (Rhodopsin)ELISA Kit
Purpose
Our Human RHO (Rhodopsin) ELISA kit is to assay Rhodopsin levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Rhodopsin. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Rhodopsin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Rhodopsin and HRP conjugated Rhodopsin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rhodopsin. You can calculate the concentration of Rhodopsin in the samples by comparing the OD of the samples to the standard curve.
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