Summary:

 

Purpose

 

Our Quick Step Human hypoxia-inducible factor 1beta (HIF-1beta)elisa Kit is to assay HIF-1beta levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to HIF-1beta. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for HIF-1beta is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HIF-1beta and HRP conjugated HIF-1beta Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HIF-1beta. You can calculate the concentration of HIF-1beta in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1924Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.059	0.0560
		0.053
S1	360pg/ml	1.975	1.9695
		1.964
S2	240pg/ml	1.388	1.3800
		1.372
S3	120pg/ml	0.705	0.6995
		0.694
S4	60pg/ml	0.383	0.3800
		0.377
S5	30pg/ml	0.197	0.2010
		0.205

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