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Quick Step Mouse Kidney injury molecule 1 ,Kim-1 ELISA Kit
Quick Step Mouse Kidney injury molecule 1 ,Kim-1 ELISA Kit
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  • NO.:QS0339Mo
    Sensitivity:2.2 pg/ml
    Assay range:8 pg/ml -500 pg/ml
    Standard:720 pg/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
  • Goods click count:64
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Details

 

Summary:

 

Purpose

 

Our Quick Step Mouse Kidney injury molecule 1,Kim-1 ELISA Kit is to assay Kim-1 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to Kim-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for Kim-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Kim-1 and HRP conjugated Kim-1 Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Kim-1. You can calculate the concentration of Kim-1 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0339Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0pg/ml

0.052

0.0530

0.054

S1

480pg/ml

1.969

1.9715

1.974

S2

320pg/ml

1.272

1.2705

1.269

S3

160pg/ml

0.785

0.7885

0.792

S4

80pg/ml

0.474

0.4785

0.483

S5

40pg/ml

0.215

0.2190

0.223

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