Summary:

 

Purpose

 

Our Quick Step Mouse Osteopontin,OPN ELISA Kit is to assay OPN levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to OPN. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for OPN is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain OPN and HRP conjugated OPN Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of OPN. You can calculate the concentration of OPN in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0418Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.052	0.0530
		0.054
S1	480pg/ml	1.969	1.9715
		1.974
S2	320pg/ml	1.272	1.2705
		1.269
S3	160pg/ml	0.785	0.7885
		0.792
S4	80pg/ml	0.474	0.4785
		0.483
S5	40pg/ml	0.215	0.2190
		0.223

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