Summary:

 

Purpose

 

Our Quick Step Mouse Glucose-dependent insulin-releasing polypeptide,GIP ELISA Kit is to assay GIP levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GIP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for GIP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GIP and HRP conjugated GIP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GIP. You can calculate the concentration of GIP in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0238Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0nmol/L	0.043	0.0405
		0.038
S1	90nmol/L	1.954	1.9615
		1.969
S2	60nmol/L	1.318	1.3305
		1.343
S3	30nmol/L	0.683	0.6910
		0.699
S4	15nmol/L	0.391	0.3865
		0.382
S5	7.5nmol/L	0.194	0.1910
		0.188

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