Summary:

 

Purpose

 

Our Quick Step Mouse hydroxyproline,Hyp ELISA Kit is to assay Hyp levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Hyp. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Hyp is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Hyp and HRP conjugated Hyp antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Hyp. You can calculate the concentration of Hyp in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0278Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.043	0.0405
		0.038
S1	90ng/ml	1.954	1.9615
		1.969
S2	60ng/ml	1.318	1.3305
		1.343
S3	30ng/ml	0.683	0.6910
		0.699
S4	15ng/ml	0.391	0.3865
		0.382
S5	7.5ng/ml	0.194	0.1910
		0.188

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