Summary:

 

Purpose

 

Our Quick Step Human adrenergic alpha-1A receptor,ADRA1A ELISA Kit is to assay ADRA1A levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an 抗体QS0074Hu specific to ADRA1A. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific 抗体QS0074Hu. Then a Horseradish Peroxidase (HRP)- conjugated 抗体QS0074Hu specific for ADRA1A is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ADRA1A and HRP conjugated ADRA1A 抗体QS0074Hu will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ADRA1A. You can calculate the concentration of ADRA1A in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0074Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.040	0.0450
		0.050
S1	4.8ng/ml	1.958	1.9555
		1.953
S2	3.2ng/ml	1.308	1.3090
		1.310
S3	1.6ng/ml	0.698	0.7045
		0.711
S4	0.8ng/ml	0.397	0.3935
		0.390
S5	0.4ng/ml	0.209	0.2075
		0.206

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