Summary:

 

Purpose

 

Our Quick Step Mouse Lipid Peroxide (LPO)elisa Kit is to assay LPO levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an 抗体QS0967Mo specific to LPO. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific 抗体QS0967Mo. Then a Horseradish Peroxidase (HRP)- conjugated 抗体QS0967Mo specific for LPO is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain LPO and HRP conjugated LPO 抗体QS0967Mo will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of LPO. You can calculate the concentration of LPO in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0967Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μmol/L	0.040	0.0450
		0.050
S1	4.8μmol/L	1.958	1.9555
		1.953
S2	3.2μmol/L	1.308	1.3090
		1.310
S3	1.6μmol/L	0.698	0.7045
		0.711
S4	0.8μmol/L	0.397	0.3935
		0.390
S5	0.4μmol/L	0.209	0.2075
		0.206

Cartpieces

• 
• 1

  1Delete

• 
• 8

  8Delete

• 
• Q

  QDelete

• 
• Q

  QDelete

• 
• +

  +Delete

• 
• 1

  1Delete

• 
• 2

  2Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• Q

  QDelete

• Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• Q

  QDelete

• 
• 8

  8Delete

• 
• Q

  QDelete

Totalgoods,subtotals:￥Checkout