Summary:

 

Purpose

 

Our Quick Step Mouse 25-Dihydroxy vitamin D3,HVD3 ELISA Kit is to assay HVD3 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HVD3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HVD3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HVD3 and HRP conjugated HVD3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HVD3. You can calculate the concentration of HVD3 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0005Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.048	0.0465
		0.045
S1	18ng/ml	1.973	1.9760
		1.979
S2	12ng/ml	1.363	1.3675
		1.372
S3	6ng/ml	0.725	0.7220
		0.719
S4	3ng/ml	0.388	0.3860
		0.384
S5	1.5ng/ml	0.187	0.1895
		0.192

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