Summary:

 

Purpose

 

Our Quick Step Rat Glutamate ELISA Kit is to assay Glutamate levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Glutamate. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Glutamate is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Glutamate and HRP conjugated Glutamate antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Glutamate. You can calculate the concentration of Glutamate in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1393Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μmol/L	0.043	0.0460
		0.049
S1	9μmol/L	1.933	1.9385
		1.944
S2	6μmol/L	1.394	1.4015
		1.409
S3	3μmol/L	0.713	0.7095
		0.706
S4	1.5μmol/L	0.400	0.3970
		0.394
S5	0.75μmol/L	0.206	0.2125
		0.219

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