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Quick Step Rat N-acetyl-beta-D-glucosaminidase (NAG)ELISA Kit
Quick Step Rat N-acetyl-beta-D-glucosaminidase (NAG)ELISA Kit
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  • NO.:QS0513Ra
    Sensitivity:9.2pg/ml
    Assay range:45pg/ml-4000pg/ml
    Standard:5400pg/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
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Details

Purpose

Our Quick Step Rat N-acetyl-beta-D-glucosaminidase (NAG)ELISA Kit is to assay NAG levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NAG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for NAG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NAG and HRP conjugated NAG antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NAG. You can calculate the concentration of NAG in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 

The standard curve of QS0513Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0pg/ml

0.052

0.0460

0.040

S1

3600pg/ml

1.912

1.9075

1.903

S2

2400pg/ml

1.388

1.3840

1.380

S3

1200pg/ml

0.708

0.7100

0.712

S4

600pg/ml

0.403

0.4000

0.397

S5

300pg/ml

0.189

0.1960

0.203 

 

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