Determination of Significance：

Acetolactate Synthase (ALS, EC 4.1.3.18), also known as Acetohydroxyacid synthase (AHAS). ALS is present in plant growth and is a key enzyme in the induction of the biosynthesis of the branched-chain amino acids (Ile, Leu, and Val) during the The key enzyme in the first stage of biosynthesis of branched chain amino acids (Ile, Leu, Val).

Measurement Principle：

ALS catalyzes the formation of acetolactate from pyruvate, which is decarboxylated under certain conditions to form acetoin, which reacts with creatine and α-naphthol under alkaline conditions to form a red substance with a maximum absorbance value at 525 nm.

Self Provided：

Microplate Reader/spectrophotometer, water bath/incubator, benchtop centrifuge, balance, adjustable pipettes, micro glass cuvettes/96-well plates, mortar and homogenizer, distilled water, ice, anhydrous ethanol and ammonium sulphate.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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