Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA in vivo and is A key enzyme in the synthesis of fatty acids and many secondary metabolites. The activity of ACC determines the synthesis speed and oil content of fatty acids to some extent.

ACC can catalyze acetyl CoA, NaHCO3 and ATP to produce malonyl-CoA, ADP and inorganic phosphorus, and pyruvate kinase and lactate dehydrogenase further catalyze NADH to produce NAD+. The decline rate of NADH at 340nm can reflect ACC activity.

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