Isocitric Acid, an isomer of citric acid, is found in large amounts in many fruits and vegetables and in foods made from these ingredients. What organisms can use is type D isocitric acid, a component of the tricarboxylic acid cycle.

The isocitric acid is converted to A-ketoglutaric acid by isocitric dehydrogenase (ICDH, EC1.1.1.41), while NAD (P) is reduced to NAD (P) H, from which the isocitric acid content can be calculated.

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