Determination of Significance：

Hemoglobin (Hb) is a cofactor-containing protein with heme as prosthetic group, which plays an important role in transporting and storing oxygen. It is comprised of two alpha and two beta chains. Each chain has a cyclic tetrapyrrole heme containing an iron atom. Oxygen binds to iron atoms and is transported in the blood.

Measurement Principle：

The improved aqueous alkaline method is used to measure Hb concentration. Hb could react with working solution to form colored compound, which has an absorption peak at 400 nm. Changes of the absorbance at 400nm could be measured to reflect Hb content.

Self Provided：

Spectrophotometer, desk centrifuge, balance, transferpettor, 1mL glass cuvette, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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