Determination of Significance：

Iron is one of the essential trace elements in human body, which is the main component of hemoglobin, myoglobin, cytochrome and other enzyme systems. Iron can assist in the transport of oxygen and promote fat oxidation. Iron deficiency can easily cause anemia, metabolic disorders, and affect the immune function of the body.

Measurement Principle：

Fe³⁺ is reduced by hydroxylamine hydrochloride to Fe²⁺. Fe²⁺ could react with Phenanthroline Monohydrate to form a kind of orange compound under weak acid condition, which has an absorption peak at 510 nm. According measure absorbance at 520 nm can reflect cell iron concentration.

Self Provided：

Spectrophotometer, desk centrifuge, adjustable pipette, 1mL glass cuvette, cell ultrasonic crusher, ice, distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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