Determination of Significance：

Pyruvate plays an important role in the metabolism of glucose, fatty acids and amino acids through acetyl CoA.

Measurement Principle：

At pH=7.5, pyruvate reacts with NADH catalyzed by LDH to produce NAD+ and lactic acid. Under 1-mPMS, WST-1 reacts with NADH to produce water-soluble Formazan. Pyruvate content can be calculated by detecting the absorption value at 450nm.

Self Provided：

Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath/ constant temperature incubator, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate, ultrasonic cleaning instrument, 30-50 mesh sieve, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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