Determination of Significance：

Carbonic anhydrase (CA, EC4.2.1.1) is a metalloenzyme with Zn²⁺ as the active center, which can efficiently catalyze the reversible hydration reaction of CO2: CO2+H2O ?HCO3-+H+ at a rate 107 times higher than that under natural conditions, which is one of the fastest known enzymes. It is one of the fastest known enzymes.

Measurement Principle：

Carbonic anhydrase can catalyze the reaction of p-nitrophenyl acetate to form p-nitrophenol, and the rate of increase of absorbance value at 405 nm reflects the activity of carbonic anhydrase.

Self Provided：

Spectrophotometer, centrifuge, water bath/incubator, adjustable pipette, 1mL glass cuvette, mortar/homogenizer/cell sonicator, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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