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Cerebrospinal fluid Total Protein Assay Kit (Turbidity and colorimetry method)
Cerebrospinal fluid Total Protein Assay Kit (Turbidity and colorimetry method)
脑脊液总蛋白试剂盒 (浊度和比色法)
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Cerebro-Spinal Fluid (CSF) is a colorless transparent fluid that exists in the cerebral ventricle, subarachnoid space and central canal of the spinal cord. It is produced by the choroid plexus in the cerebral ventricle. It has similar properties to plasma and lymph. Normal cerebrospinal fluid has a certain chemical composition and pressure, which plays an important role in maintaining the relative stability of intracranial pressure. When the central nervous system is damaged, the detection of cerebrospinal fluid becomes an important auxiliary diagnostic method. Total Protein (TP) is composed of albumin and globulin. The determination of TP in biological fluids (serum, urine, cerebrospinal fluid) is generally based on the following two assumptions: 1. All protein molecules are composed of pure peptides with a mass percentage of nitrogen content of 16%; 2. There are hundreds of protein molecules in body fluids, and each molecule has very similar characteristics to the determination reaction. At present, the commonly used methods for the detection of total protein are: biuret method, ultraviolet spectrophotometry, dye binding method, Kjeldberg nitrogen method, precipitation method, etc.

The detection principle of this kit is that the protein in cerebrospinal fluid and sulfosalicylic acid interact to form precipitation, and the turbidity is measured by turbidimetry. Compared with the standard solution with the same treatment, the content of protein in the sample is calculated. It can be used for the determination of total protein content in human or animal cerebrospinal fluid samples, but it is easily affected by surfactant.


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