Determination of Significance：

Fluoride resistant acid phosphatase (FRAP) is a type of acid phosphatase. FRAP is mainly found in the lysosomes of most cells, the prostate gland, brain, liver, spleen, and platelets.

Measurement Principle：

The activity of fluoride-resistant acid phosphatase is not inhibited by fluoride ions, whereas the activity of other acid phosphatases is inhibited by fluoride ions. Under acidic conditions, the fluoride-resistant ion acid phosphatase catalyzes the production of p-nitrophenol from PNPP. The p-nitrophenol appears yellow under alkaline conditions and can be detected at 400 nm absorbance. The darker the yellow color of the product, the higher the activity of the fluorine-resistant ion acid phosphatase, and vice versa, the lower the enzyme activity.

Self Provided：

Spectrophotometer/microplate reader, centrifuge, water bath/incubator, adjustable pipette, Micro glass cuvettes/96 well plates, mortar/homogenizer/cell sonicator, ice and distilled water

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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