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Arginase Activity Assay Kit
Arginase Activity Assay Kit
精氨酸酶 活性 检测试剂盒
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Determination of Significance:

Arginase, also known as L-arginine urea hydrolase or L-arginine amidinyl hydrolase, is a manganese metalloenzyme. Arginase is found in bacteria, yeast, plants, invertebrates and vertebrates, and is thought to have first appeared in bacteria. The main function of arginase in microorganisms is probably to participate in maintaining the dynamic balance of L-arginine and in regulating a variety of important metabolic processes.

Measurement Principle:

Arginase catabolic L-arginine into L-ornithine and urea, urea reacts with α-isonitrosopropiophenone to produce a derivative with an absorption peak at 560 nm. By measuring the production of urea, the size of the arginine activity can be calculated

Self Provided:

Spectrophotometer, low temperature centrifuge, adjustable transferpettor, water bath/ constant temperature incubator, 1ml glass cuvette, mortar/homogenizer/cell ultrasonicator , ice and distilled water.

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