Determination of Significance：

Methemoglobin (MetHb) is the oxidation of hemoglobin (Hb), which ferrous ions are oxidized to ferric ions in hemoglobin. Hemoglobin can transport oxygen, but methemoglobin cannot. MetHb content can reflect oxygen carrying capacity of blood and red blood cell substitutes. It is important to detect MetHb content for diagnosis of methemoglobinemia and development of red blood cell substitutes.

Measurement Principle：

Hemoglobin binds to oxygen to form oxyhemoglobin and the latter is deoxidized to form deoxyhemoglobin. Rates of each component mass concentration can be calculated by their extinction coefficients and absorptions at 560nm, 576nm and 630nm. After measuring total Hb content, MetHb content of sample can be calculated by the rates of each component.

Self Provided：

Spectrophotometer/Microplate reader, desk centrifuge, balance, transferpettor, micro glass cuvette/96 well plate, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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