Dicotyledonous plants and non-gramineous monocotyledonous plants obtain iron from soil using a highly efficient activation and absorption mechanism of iron chelation reduction. Iron is reduced to iron bivalent before it can be absorbed and utilized by plants. When iron is sufficient, ferric oxidoreductase in plant roots reduces iron in Fe(III) -chelates and transports the resulting Fe2+ through the plasma membrane into root cells.

Ferric chelate reductase (FCR, EC1.16.1.7) catalyzes the reduction of Fe3+ to Fe2+, and Fe2+ and phenoxazine form purple complexes with characteristic absorption peaks at 562nm.

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