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Calcium Assay Kit (methyl thymol Blue complexed copper microplate method)
Calcium Assay Kit (methyl thymol Blue complexed copper microplate method)
钙试剂盒 (甲基百里酚蓝络合铜微量板法)
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Calcium is a metal element, which is a silver-white crystal at room temperature. Animal bones, clam shells and egg shells all contain calcium carbonate. The detection of calcium content in living organisms is mainly achieved by detecting the concentration of calcium ions. At present, the commonly used dyes include o-cresolphthalide complex ketone (OCPC), azo arsenic Ⅲ, methyl thymol blue (MTB), etc.            

The kit uses calcium ions in solution under alkaline conditions to combine with OCPC to form a purplish red complex, adding magnesium ion chelator to remove magnesium ion background interference, detecting the absorbance at 575nm by microplate reader, and calculating the total calcium content according to the formula; The advantages of this method are as follows: 1. It is a routine method of serum calcium detection recommended by the world; 2. Single reagent, easy to operate; 3. High sensitivity; 4. Stable color development 5. Small sample size.


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