Determination of Significance：

Reactive oxygen species such as superoxide anion in the organism have the functions of immunity and signal transduction, but if they accumulate too much, they will cause damage to cell membranes and biological macromolecules, resulting in abnormal metabolism of cells and tissues in the body, thereby causing a variety of diseases.

Measurement Principle：

The AP-TEMED system produces superoxide anion, which reacts with hydroxylamine hydrochloride to form NO^2-, which reacts with p-aminobenzenesulfonamide and α-naphthylamine to form red azo compounds, with a characteristic absorption peak at 530nm. The scavenging ability of oxygen anions was negatively correlated with the absorbance at 530 nm.

Self Provided：

Spectrophotometer/microplate reader, desk centrifuge, Constant temperature incubator/water bath, pipette, micro glass cuvette/96 well plate, mortar/homogenizer/sonicator, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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