Determination of Significance：

Pyrroline-5-carboxylate reductase (P5CR) is an important housekeeping protein widely found in prokaryotes and eukaryotes. In the presence of NAD(P)H, pyrroline-5-carboxylic acid (P5C) is converted to proline by pyrroline-5-carboxylate reductase, and P5CR has also been found to be involved in the metabolism of thioproline in E. coli.

Measurement Principle：

Thioproline is dehydrogenated by pyrroline-5-carboxylate reductase and is accompanied by the conversion of NAD to NADH. In the presence of 1-mPMS, WST-1 reacts with NADH to produce water-soluble formazan with a characteristic absorption peak at 450 nm.

Self Provided：

Spectrophotometer, water bath/constant incubator, adjustable pipette, analytical balance, mortar/homogenizer/cell sonicator , 1mL glass cuvette, distilled water and ice.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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