Determination of Significance：

Alpha-ketoglutarate (α-KG) is an important metabolic intermediate in the tricarboxylic acid cycle and a key link between intracellular carbon and nitrogen metabolism. α-KG, as a short-chain carboxylic acid molecule, is a precursor to many important amino acids such as glutamine and glutamate, and is not only directly involved in energy supply, but also participates in a variety of intracellular chemical reactions and has a variety of physiological effects.

Measurement Principle：

GDH catalyses the formation of glutamate and NAD+ from NH4+, α-ketoglutarate and NADH, causing a decrease in absorbance at 340nm. The α-ketoglutarate content was calculated by measuring the change in NADH.

Self Provided：

Ultraviolet spectrophotometer/ microplate reader, centrifuge, water bath/ heating dry baths / constant temperature incubator, analytical balance, adjustable pipette, micro quartz cuvettes/96-well UV plates, mortar/homogeniser/cell ultrasonicator, distilled water and ice.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Cartpieces

• 
• 1

  1Delete

• 
• 4

  4Delete

• 
• A

  ADelete

• 
• �

  �Delete

• 
• +

  +Delete

• 
• 9

  9Delete

• 
• 2

  2Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 2

  2Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• �

  �Delete

• 
• �

  �Delete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 2

  2Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• �

  �Delete

• 
• 4

  4Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout