Determination of Significance：

Lactate dehydrogenase (LDH) is the terminal enzyme of the glycolysis pathway which is widely found in animals, plants, microorganisms and cultured cells. LDH catalyzes the reversible conversion of lactate to pyruvic acid with the reduction of NAD+ to NADH and vice versa. According to the different configuration of catalytic substrate, it could be divided into D-lactate dehydrogenase (D-LDH, EC1.1.1.28) and L-lactate dehydrogenase (L-LDH, EC1.1.1.27).

Measurement Principle：

NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of D-LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.

Self Provided：

Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer/cell ultrasonic crusher, ice, distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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