Determination of Significance：

Monoamine oxidase (EC1.4.3.4) mainly exists in various organs of vertebrates, especially secretory glands, brain, and liver. In the buds of invertebrates, beans and other plants, there are also catalyzed monoamine metabolisms, which are low in content and have important physiological functions, and the activity of monoamine oxidase can reflect the degree of liver fibrosis. In addition, the abnormal activity of monoamine oxidase leads to disorder of monoamine neurotransmitter in the cell, which leads to a variety of diseases.

Measurement Principle：

Monoamine oxidase catalyzes the deamination of monoamine substrates to generate corresponding aldehydes, which are further oxidized to acid; the substrate has a characteristic absorption peak at 360nm. Therefore, the monoamine oxidase activity can be calculated by measuring the rate of decrease at 360 nm.

Self Provided：

Spectrophotometer, Centrifuge, Water-bath/Constant temperature incubator, 1 mL quartz cuvette, Distilled water, Adjustable pipette.

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