Summary:

 

Purpose

Our Quick Step Rat Phoshorylation (Akt)ELISA Kit is to assay Akt levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Akt. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Akt is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Akt and HRP conjugated Akt antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Akt. You can calculate the concentration of Akt in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS1882Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.052	0.0460
		0.040
S1	3600pg/ml	1.912	1.9075
		1.903
S2	2400pg/ml	1.388	1.3840
		1.380
S3	1200pg/ml	0.708	0.7100
		0.712
S4	600pg/ml	0.403	0.4000
		0.397
S5	300pg/ml	0.189	0.1960
		0.203

 

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