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Summary:
Purpose
Our Quick Step Rat Free Tri-iodothyronine Indes (Free-T3)ELISA Kit is to assay Free-T3 levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Free-T3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Free-T3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Free-T3 and HRP conjugated Free-T3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Free-T3. You can calculate the concentration of Free-T3 in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QS0296Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Standard
Concentration
OD Value
Average OD Value
Blank Well
0.00pmol/L
0.053
0.0525
0.052
S1
6.00 pmol/L
1.951
1.9440
1.937
S2
4.00 pmol/L
1.311
1.3150
1.319
S3
2.00 pmol/L
0.718
0.7220
0.726
S4
1.00 pmol/L
0.391
0.3890
0.387
S5
0.50 pmol/L
0.213
0.2090
0.205
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