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Summary:
Purpose
Our Quick Step Rat Hypoxia-Inducible Factor 1 Beta (HIF-1β)ELISA kit is to assay HIF-1β levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HIF-1β. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HIF-1β is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HIF-1β and HRP conjugated HIF-1β antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HIF-1β. You can calculate the concentration of HIF-1β in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QS1886Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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Standard
Concentration
OD Value
Average OD Value
Blank Well
0pg/ml
0.049
0.0440
0.039
S1
1800pg/ml
1.956
1.9530
1.950
S2
1200pg/ml
1.389
1.3910
1.393
S3
600pg/ml
0.709
0.7135
0.718
S4
300pg/ml
0.390
0.3875
0.385
S5
150pg/ml
0.195
0.2000
0.205
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