Summary:

 

Purpose

Our Quick Step Rat Hypoxia-Inducible Factor 1 Beta (HIF-1β)ELISA kit is to assay HIF-1β levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HIF-1β. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HIF-1β is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HIF-1β and HRP conjugated HIF-1β antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HIF-1β. You can calculate the concentration of HIF-1β in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS1886Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.049	0.0440
		0.039
S1	1800pg/ml	1.956	1.9530
		1.950
S2	1200pg/ml	1.389	1.3910
		1.393
S3	600pg/ml	0.709	0.7135
		0.718
S4	300pg/ml	0.390	0.3875
		0.385
S5	150pg/ml	0.195	0.2000
		0.205

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